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Table 1 Pyrosequencing primers used to genotype polymorphisms in MSP-119.

From: A high-throughput method for quantifying alleles and haplotypes of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-1 19 kDa

SNP Primer
Location Nucleotides Amino Acids
1644 G/C E/Q 5'-GCGTAAAAAAACAATGTC-3'
1691 A/C K/T 5'-GTGATGCAGATGCCA-3'
1699a G/A S/N 5'-CCGAAGAAGATTCAGGTA-3'
1700a G/A S/N  
1701a G/A G/R  
1716b C/T L/F 5'-TCACATGTGAATGTACTAAA-3'
  1. aSNPs at these three positions are genotyped in the same Pyrosequencing reaction.
  2. b Primer sits down prior to position 1711 where there is a rare polymorphism not considered in this analysis.
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