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Table 1 Pyrosequencing primers used to genotype polymorphisms in MSP-119.

From: A high-throughput method for quantifying alleles and haplotypes of the malaria vaccine candidate Plasmodium falciparum merozoite surface protein-1 19 kDa

SNP

Primer

Location

Nucleotides

Amino Acids

1644

G/C

E/Q

5'-GCGTAAAAAAACAATGTC-3'

1691

A/C

K/T

5'-GTGATGCAGATGCCA-3'

1699a

G/A

S/N

5'-CCGAAGAAGATTCAGGTA-3'

1700a

G/A

S/N

 

1701a

G/A

G/R

 

1716b

C/T

L/F

5'-TCACATGTGAATGTACTAAA-3'

  1. aSNPs at these three positions are genotyped in the same Pyrosequencing reaction.
  2. b Primer sits down prior to position 1711 where there is a rare polymorphism not considered in this analysis.
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Malaria Journal

ISSN: 1475-2875

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