Skip to main content

Table 1 PCR-RFLP analysis of msp-3α gene in 50 northern and 94 southern P. vivax clinical isolates from Iran. B = Belem Type 1; S =Salvador I Type 2; R = Recombinant Type 3 [20]. N = northern sample, S = southern sample. Different allelic variants obtained fromsequencing analysis of Iranian Pvmsp-1 gene grouped to Type 1 (B1-B9), Type 2 (S1-S16) and Type 3 (R1-R5). Within the same Typeall samples showed 100% similarity at both nucleotides and protein levels [20]. However, applying PCR-RFLP for genetic analysis ofidentical P. vivax samples by using Pvmsp-3α marker showed allelic variants within each Type.

From: Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax

Isolate Group Amplified fragment size Number of the allelic variant based on RFLP method
  Type A(1800 bp) Type B(1500 bp) Type C(1200 bp) Hha I Alu I
B1N 3/37 (8.1%) 2/37(5.4%) 31/37(83.8%) 4 4
B1S - - 1/37 (2.7%) 1 1
B2N - - 2/2 (100) 1 1
B2S - - - - -
B3N - - - - -
B3S 3/3 (100%) - - 2 2
B4N - - - - -
B4S 1/1 (100%) - - 1 1
B5N - - - - -
B5S - 1/1 (100%) - 1 1
B6N - - - - -
B6S 2/2 (100%) - - 1 1
B7N - - - - -
B7S - - 2/2 (100%) 2 2
B8N - - - - -
B8S 3/3 (100%) - - 1 1
B9N - - - - -
B9S 3/3 (100%) - - 2 2
S1N 5/23 (21.7%) 1/23 (4.3%) - 3 3
S1S 13/23 (56.5%) 2/23 (8.7%) 2/23 (8.7%) 7 7
S2N 3/9 (34%) - 1/9 (11%) 2 2
S2S 1/9 (11%) 2/9 (22%) 2/9 (22%) 3 3
S3N - - 1/1 (100%) 1 1
S3S - - - - -
S4N - - - - -
S4S 1/1 (100%) - - 1 1
S5N - - - - -
S5S 2/2 (100%) - - 1 1
S6N - - - - -
S6S 1/1 (100%) - - 1 1
S7N - - - - -
S7S 1/1 (100%) - - 1 1
S8N - - - - -
S8S 2/2 (100%) - - 1 1
S9N - - - - -
S9S 5/5 (100%) - - 1 1
S10N - - - - -
S10S 3/3 (100%) - - 2 2
S11N - - - - -
S11S 1/1 (100%) - - 1 1
S12N - - - - -
S12S 2/9 (22.2%) 1/9 (11.1%) 6/9 (66.7%) 2 2
S13N - - - - -
S13S 1/2 (50%) - 1/2 (50%) 2 2
S14N - - - - -
S14S 1/1 (100%) - - 1 1
S15N - - - - -
S15S 2/2 (100%) - - 2 2
S16N - - - - -
S16S 1/1 (100%) - - 1 1
R1N 1/3 (33.4%) - - 1 1
R1S 2/3 (66.6%) - - 2 2
R2N - - - - -
R2S - - - - -
R3N - - - - -
R3S 2/4 (50%) 1/4 (25%) 1/4 (25%) 3 3
R4N - - - - -
R4S 2/2 (100%) - - 2 2
R5N - - - - -
R5S 13/17 (76.5%) - 4/17 (23.5%) 4 4
Total (144) 80 (55.5%) 10 (7%) 54 (37.5%) 61 61
\