(A). Autoradiograph of proteins from trophozoite stage iRBC infected with 3D7, A4 and ItG. At 20 hours after invasion, parasites were metabolic labelled with 50 μCi/ml [35S] methionine for 4 hours. iRBC ghosts were separated from the free parasite fraction and run on pH 4-7 IEF strips followed by 12% SDS-PAGE. Gels were stained with Coomassie Blue, dried and exposed to x-ray film. Marked circles show proteins with at least three fold changes in expression compared with 3D7. High quality tif files showing the original 2D gels of metabolic labelled iRBC ghosts from parasite lines 3D7 (Additional file 17), A4 (Additional file 18) and ItG (Additional file 19) available. (B). Enlarged images of parts of Fig.6A showing significant changes in the protein profiles. Arrows and circles indicate the relative positions of the spots in different lines.