Comparison of three molecular methods for the detection and speciation of Plasmodium vivax and Plasmodium falciparum

Table 1 Primer Sequences

PCR	Primer Name	Primer Sequence
Nested – 1 ^st round	rPLU6	TTA AAA TTG TTG CAG TTA AAA CG
	rPLU5	CCT GTT GTT GCC TTA AAC TTC
Nested – 2 ^nd round	rFAL1	TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT
	rFAL2	ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC
	rVIV1	CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC
	rVIV2	ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA
	rMAL1	ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC
	rMAL2	AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA
	rOVA1	ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA
	rOVA2	GGA AAA GGA CAC ATT AAT TGT ATC CTA GTG
Multiplex	Reverse	GTA TCT GAT CGT CTT CAC TCCC
	PF	AAC AGA CGG GTA GTC ATG ATT GAG
	PV	CGG CTT GGA AGT CCT TGT
	PO	CTG TTC TTT GCA TTC CTT ATG C
	PM	CGT TAA GAA TAA ACG CCA AGC
Real-time	Primer 1	TAA CGA ACG AGA TCT TAA
	Primer 2	GTT CCT CTA AGA AGC TTT

Primer sequences for 1^st round nested PCR, 2^nd round nested PCR, multiplex PCR and real-time PCR.