Nested – 1
st
round
|
rPLU6
|
TTA AAA TTG TTG CAG TTA AAA CG
|
|
rPLU5
|
CCT GTT GTT GCC TTA AAC TTC
|
Nested – 2
nd
round
|
rFAL1
|
TTA AAC TGG TTT GGG AAA ACC AAA TAT ATT
|
|
rFAL2
|
ACA CAA TGA ACT CAA TCA TGA CTA CCC GTC
|
|
rVIV1
|
CGC TTC TAG CTT AAT CCA CAT AAC TGA TAC
|
|
rVIV2
|
ACT TCC AAG CCG AAG CAA AGA AAG TCC TTA
|
|
rMAL1
|
ATA ACA TAG TTG TAC GTT AAG AAT AAC CGC
|
|
rMAL2
|
AAA ATT CCC ATG CAT AAA AAA TTA TAC AAA
|
|
rOVA1
|
ATC TCT TTT GCT ATT TTT TAG TAT TGG AGA
|
|
rOVA2
|
GGA AAA GGA CAC ATT AAT TGT ATC CTA GTG
|
Multiplex
|
Reverse
|
GTA TCT GAT CGT CTT CAC TCCC
|
|
PF
|
AAC AGA CGG GTA GTC ATG ATT GAG
|
|
PV
|
CGG CTT GGA AGT CCT TGT
|
|
PO
|
CTG TTC TTT GCA TTC CTT ATG C
|
|
PM
|
CGT TAA GAA TAA ACG CCA AGC
|
Real-time
|
Primer 1
|
TAA CGA ACG AGA TCT TAA
|
|
Primer 2
|
GTT CCT CTA AGA AGC TTT
|