Comparison of three molecular methods for the detection and speciation of Plasmodium vivax and Plasmodium falciparum

Table 2 Speciation results of all samples

Percentage of total isolates (n = 136)	PCR			Molecular Consensus	Microscopy
	Multiplex	Nested	Real-time
23.5% (32)	Pf	Pf	Pf	Pf	Pf
0.7% (1)	Pf	Pf + Pv	Pf + Pv	Pf+ Pv	Pf
34.6% (47)	Pv	Pv	Pv	Pv	Pv
2.2% (3)	Neg	Neg	Neg	Neg	Pv
1.5% (2)	Pf	Pf	Pf	Pf	Pv
1.5% (2)	Pf	Pf	Pf	Pf	Pf + Pv
1.5% (2)	Pf	Pf + Pv	Pf + Pv	Pf + Pv	Pf + Pv
30.9% (42)	Neg	Neg	Neg	Neg	Neg
2.2% (3)	Pv	Pv	Pv	Pv	Neg
0.7% (1)	Pf	Pf	Pf	Pf	Neg
0.7% (1)	Pv	Pf + Pv	Pv	Pv	Neg

Results are P. falciparum (Pf), P. vivax (Pv) or negative (Neg). Samples are grouped by similar results by all techniques, there are 11 result combinations and are represented in each of the tables rows. Molecular consensus in column five is was determined when two or more of the three molecular techniques were alike.

ISSN: 1475-2875