Figure 3
(A) Western blot showing different isoforms of enolase associated with different sub-cellular fractions of P. yoelii. 0.8–0.9 mg of acetone powder prepared from cytosol, nuclei, membranes or cytoskeletal components were analysed by two dimensional gel electrophoresis (2DIGE) and transferred to a nitrocellulose membrane. Blots were probed with anti-r-Pfen antibodies. (B) Detection of phosphorylated enolase in P. yoelii cell extract. Fe+3-iminodiaceticacidagarose beads were used to purify phospho-proteome. Whole cell extract (lane 1) and purified phosphoprotome (lane 2) were analysed on 12% SDS-PAGE and gel was silver stained. (C) Western blot of gel in (B) probed with anti-r-Pfen antibodies.