From: Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative study
Primer Pair | Primer | Length (bp) | Tm* (°C) | Primer Sequence (5' to 3') | Mutagenesis method |
---|---|---|---|---|---|
P1 | A3consF | 43 | 78 | gctttatgatagtagtgatgctgataattataataaggaaagc | QuickChange™ site-directed method |
A3consR | 43 | 78 | gctttccttattataattatcagcatcactactatcataaagc | ||
P2 | A3overF | 49 | 79 | gatagtagtgatgctgat ↓ aattataataaggagagctttttatataatg | Overlapping primer method [19] |
A3overR | 55 | 80 | gctttccttattataatt ↓ atcagcatcactactatcataaagctttaaattatcc | ||
P3 | A3reF | 27 | 73(62) | cgcggatcc aattataataaggaaagc | ExSite™, inverse [18] and RE-mediated inverse PCR methods |
A3reR | 34 | 79(69) | cgcggatcc atcagcatcactactatcataaagc | ||
P4 | PdxkF | 34 | 78(67) | cgcggatcc aatctaaattttctttgggtatgtg | RE-mediated inverse PCR method |
PdxkR | 38 | 79(67) | cgcggatcc tttccttcttaattcaagtatatttttgg |