Table 1 Fluorescence staining of pRBCs with different fluorescent dyes with differing absorption and emission spectra
From: The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria
Fluorescent dye | Spectra Em/Ex (nm) | Detection filter* | Intensity of fluorescent staining†|
|---|---|---|---|
SYBR Green 1 | 497/520 | 2 | ++++ |
Ethidium monoazide (EMA) | 504/600 | 3 | Â |
non photoactivated | Â | Â | +++ |
photoactivated | Â | Â | ++++ |
Yoyo-1 | 491/509 | 2 | +++ |
Acridine Orange | 502/526 | Â | Â |
Live/Dead Reduced Biohazard | Â | Â | Â |
   Viablity/Cytotoxicity Kit |  |  |  |
Component A (SYTO 10) | 484/505 | 2 | ++ |
Component B (Ethidium homodimer-2 [EthD-2]) | 520/610 | 3 | ++++ |
A+B | Â | 3 | ++ |
blue fluorescent dyes | Â | Â | Â |
SYTO 40 | 420/441 | 2 | + |
SYTO 41 | 430/454 | 2 | + |
SYTO 42 | 433/460 | 2 | + |
SYTO 43 | 436/467 | 2 | + |
SYTO 44 | 446/471 | 2 | ++++ |
SYTO 45 | 455/484 | 2 | +++ |
red fluorescent dyes | Â | Â | Â |
SYTO 17 | 621/634 | 3 | +++++ |
SYTO 59 | 622/645 | 3 | +++++ |
SYTO 60 | 652/678 | 3 | + |
SYTO 61 | 628/645 | 3 | ++++ |
SYTO 62 | 652/676 | 3 | - |
SYTO 63 | 657/673 | 3 | + |
SYTO 64 | 599/619 | 3 | +++++ |
GelRed | 500/600 | 3 | ++++ |
Hoescht 33258 | 345/487 | 1 | ++ |
DAPI | 358/461 | 1 | ++ |