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Table 1 Fluorescence staining of pRBCs with different fluorescent dyes with differing absorption and emission spectra

From: The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria

Fluorescent dye Spectra Em/Ex (nm) Detection filter* Intensity of fluorescent staining
SYBR Green 1 497/520 2 ++++
Ethidium monoazide (EMA) 504/600 3  
non photoactivated    +++
photoactivated    ++++
Yoyo-1 491/509 2 +++
Acridine Orange 502/526   
Live/Dead Reduced Biohazard    
   Viablity/Cytotoxicity Kit    
Component A (SYTO 10) 484/505 2 ++
Component B (Ethidium homodimer-2 [EthD-2]) 520/610 3 ++++
A+B   3 ++
blue fluorescent dyes    
SYTO 40 420/441 2 +
SYTO 41 430/454 2 +
SYTO 42 433/460 2 +
SYTO 43 436/467 2 +
SYTO 44 446/471 2 ++++
SYTO 45 455/484 2 +++
red fluorescent dyes    
SYTO 17 621/634 3 +++++
SYTO 59 622/645 3 +++++
SYTO 60 652/678 3 +
SYTO 61 628/645 3 ++++
SYTO 62 652/676 3 -
SYTO 63 657/673 3 +
SYTO 64 599/619 3 +++++
GelRed 500/600 3 ++++
Hoescht 33258 345/487 1 ++
DAPI 358/461 1 ++
  1. *Fluorescence filters: 1) Ex. 340–380, BA 435–485; 2) Ex 450–490/Em 520; 3) Ex 546/Em 590
  2. Intensity of fluorescence ranged from no parasite specific fluorescence (-), low (+) to very high (+++++).