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Table 1 Choice of Purification Method for high yields is dependent on the species.

From: Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies

Sample Purification
method
Average Conc.
(mg/mL)1
Mean ELISA Titer2 Recovery of Ag-
specific Igs (%)3
Purity of Ig
preparation (%)4
Stability at
4°C5
  Pre-purification 1.3 × 106 (1.9 × 105)    
Immune Rabbit CA-AS 12.3 1.4 × 106 (3.6 × 105) 100 > 95% NT
  SEP-EASE (PEG) 10.9 1.1 × 106 (9.6 × 104) 85 > 95% NT
  Protein A/G 8.9 1.1 × 106 (6.3 × 104) 84 > 95% NT
  Protein G 14.3 1.8 × 106 (2.0 × 104) 100 > 95% NT
Malaria-Exposed Human Pre-purification 1.1 × 104 (962)    
  CA-AS 12.3 1.2 × 103 (176) 11* > 95% Yes
  SEP-EASE (PEG) 14.5 1.6 × 104 (4 × 103) 100 > 95% Yes
  Protein A/G 14.4 4.8 × 103 (95) 44* >95% No
  Protein G 19.8 6.6 × 103 (2.1 × 103) 60 > 95% No
  1. Data expressed as mean (± SEM) of three independent purification experiments.
  2. 1Protein concentration was determined by the absorbance of the solution at 280 nm with an extinction coefficient of 13.5 (standard for IgG) for a 1% IgG solution (10 mg/mL)
  3. 2Mean ELISA titer for an OD405 = 1, for rabbits tested against MSP1-p42(FVO) plate antigen, for humans tested against MSP1-p42(3D7) plate antigen
  4. 3Recovery of Ag-specific Igs is based on Mean ELISA titer.
  5. 4Determined by scanning densitometry on Coomassie Blue stained gels
  6. 5Samples were stored for 24 weeks at 4°C and then tested by SDS-gelelectrophoresis, NT = not tested
  7. *A statistically significant difference between the methods in the recovery of Ag-specific Ab by ANOVA. The individual groups were isolated with a Tukey's post test (p < 0.025).