Table 2 Assay methodologies used by study participants

1A

QIAamp DNA Mini Blood Kit

Qiagen Taq DNA polymerase

Small subunit rRNA^a

1B

QIAamp DNA Mini Blood Kit

TaqMan^® universal Master MIX

Conserved ATS domain of var genes

2A

QIAamp DNA Blood kit

Eppendorf Master Mix 2.5 × kit, gel based assay

18S rRNA^b

2B

QIAamp DNA Blood kit

QuantiTectProbe PCR Kit, real-time PCR

18S rRNA^b

3A

MagNA Pure LC Total Nucleic Acid Isolation Kit

LightCycler Fast Start DNA Master SYBRGreen I

18S rRNA

3B

MagNA Pure LC Total Nucleic Acid Isolation Kit

RealArt™ Malaria LC PCR Kit

18S rRNA

4

QIAamp DNA Blood Mini Kit

MBI Taq DNA polymerase

Small subunit rRNA gene^a

5

Boom et al., 1990 [20]

RNA T7 Polymerase, AMV-RT, RNAse H, bioMerieux

18S rRNA^c

6

MagNA Pure LC DNA Isolation Kit I

LightCycler FastStart DNA Master^PLUS Hybprobe

18S rRNA

7

QIAamp DNA Mini Kit

Taqman 2 × Universal PCR Mastermix

β-tubulin^d

8

QIAamp DNA Mini Kit

TaqMan^® Universal Master Mix

18S rRNA^b

9

QIAamp DNA Mini Blood Kit

N/A

18S rRNA

10A

QIAamp DNA Mini Blood Kit

Bioline Taq Polymerase

18S rRNA^a

10B

QIAamp DNA Mini Blood Kit

Fast Start DNA Sybr Green Kit

18S rRNA

11A

Roche High Pure PCR Template Preparation Kit

Applera Taq Polymerase, Applied Biosystems

18S rRNA^e

11B

Roche High Pure PCR Template Preparation Kit

2 × Taqman Universal

18S rRNA^a

12

QIAamp DNA Mini Blood Kit

AmpliTaq Gold DNA Polymerase

18S rRNA^a

13

QIAamp DNA Blood/Mini Kit

artus Malaria PCR Kit

18S rRNA

14

High Pure PCR Template Preparation Kit

Applied Biosystems Taq Polymerase

18S rRNA^e