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Table 2 Assay methodologies used by study participants

From: Establishment of the 1st World Health Organization International Standard for Plasmodium falciparum DNA for nucleic acid amplification technique (NAT)-based assays

Lab No. Nucleic Acid Extraction Nucleic Acid Amplification Sequence Targeted
1A QIAamp DNA Mini Blood Kit Qiagen Taq DNA polymerase Small subunit rRNAa
1B QIAamp DNA Mini Blood Kit TaqMan® universal Master MIX Conserved ATS domain of var genes
2A QIAamp DNA Blood kit Eppendorf Master Mix 2.5 × kit, gel based assay 18S rRNAb
2B QIAamp DNA Blood kit QuantiTectProbe PCR Kit, real-time PCR 18S rRNAb
3A MagNA Pure LC Total Nucleic Acid Isolation Kit LightCycler Fast Start DNA Master SYBRGreen I 18S rRNA
3B MagNA Pure LC Total Nucleic Acid Isolation Kit RealArt™ Malaria LC PCR Kit 18S rRNA
4 QIAamp DNA Blood Mini Kit MBI Taq DNA polymerase Small subunit rRNA genea
5 Boom et al., 1990 [20] RNA T7 Polymerase, AMV-RT, RNAse H, bioMerieux 18S rRNAc
6 MagNA Pure LC DNA Isolation Kit I LightCycler FastStart DNA MasterPLUS Hybprobe 18S rRNA
7 QIAamp DNA Mini Kit Taqman 2 × Universal PCR Mastermix β-tubulind
8 QIAamp DNA Mini Kit TaqMan® Universal Master Mix 18S rRNAb
9 QIAamp DNA Mini Blood Kit N/A 18S rRNA
10A QIAamp DNA Mini Blood Kit Bioline Taq Polymerase 18S rRNAa
10B QIAamp DNA Mini Blood Kit Fast Start DNA Sybr Green Kit 18S rRNA
11A Roche High Pure PCR Template Preparation Kit Applera Taq Polymerase, Applied Biosystems 18S rRNAe
11B Roche High Pure PCR Template Preparation Kit 2 × Taqman Universal 18S rRNAa
12 QIAamp DNA Mini Blood Kit AmpliTaq Gold DNA Polymerase 18S rRNAa
13 QIAamp DNA Blood/Mini Kit artus Malaria PCR Kit 18S rRNA
14 High Pure PCR Template Preparation Kit Applied Biosystems Taq Polymerase 18S rRNAe
  1. Assays performed according to the following published methods a[18, 19], b [extraction method only, [20]], c[21], d[22], e[23], For other assays there were either no previously published methods or the information was not disclosed