var transcription in different adhesive 3D7 phenotypes. Transcription levels were measured by real-time PCR using primers specific for each of the 57 different var genes identified in the P. falciparum 3D7 genome. All values are presented as relative copy numbers, calibrated to the housekeeping gene seryl-tRNA synthetase (PF07_0073). Top panel: Transcription pattern of 3D7 wildtype parasites demonstrated low var gene expression in general. Second panel: 3D7 parasites selected on CHO-475 cells demonstrated expression of var genes located on chromosome 4 – PFD0995c/PFD1000c, similar to 3D7 parasites selected on CHO-K1 cells (CHO-CSA, Third panel). Fourth panel: 3D7-CD36 demonstrated expression of the PFD0615c var gene located on chromosome 4, but not the CHO-745 related var genes PFD0995c/PFD1000c. Fifth panel: 3D7-ICAM1 cells transcribed PFD0625c but also PFD0995c/PFD1000c. Sixth panel: 3D7 parasites selected on CHO-Selectin cells showed expression of PFD1000c and PFD1015c on chromosome 4. Seventh panel: 3D7-VCAM parasites transcribed PFD0995c/PFD1000c and did not show any other upregulated var gene.