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Figure 4 | Malaria Journal

Figure 4

From: The M18 aspartyl aminopeptidase of Plasmodium falciparum binds to human erythrocyte spectrin in vitro

Figure 4

Blot overlay assay of r Pf M18AAP and human erythrocyte spectrin. Slot blots containing spectrin dimers and tetramers overlaid with rPf M18AAP (+) and Tris buffer (pH 7.5) (-). Slot A1 – rPf M18AAP (positive control); A2 – bovine serum albumin (negative control); B1 – spectrin dimers; B2 – spectrin tetramers. rPf M18AAP was detected with 1:2000 Penta·His™ HRP Conjugate antibody using the SuperSignal® West Pico Chemiluminescent Substrate.

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