Figure 3

PEs surface recognition. a. Anti-var2CSA DBL mouse sera were tested for surface recognition of erythrocytes infected with parasites of the FCR3, 7G8 and HB3 strains with CSA- and CD36 binding phenotypes, respectively. Analysis was performed using flow cytometry and fluorescence microscopy. A representative example of a specific surface immunolabeling using the DBL6-1 antisera and the corresponding differential interferential contrast microscopy field are shown. Flow cytometry data shown are the normalized geometric mean fluorescence index (MFI) (± SD) obtained by subtracting the preimmune MFI value from the immune MFI value. b. Alignment of the DBL6-ε var2CSA sequences from the 3 parasite lines used to assess the antisera cross-reactivity by FACS. Conserved residues between FCR3 sequence and the other sequences are boxed with a yellow background.