Table 1 Oligonucleotide primers.
From: Characterization of two putative potassium channels in Plasmodium falciparum
Primer | Sequence (5'→ 3')a | Targetb |
|---|---|---|
P1 | cgggaattc ATGAATAATGATAATATTGGGAG | pfk1 full length (+) |
P2 | cccaagctt TCAGACTTGGTCATGCGGTTC | pfk1 full length (-) |
P3 | cgggaattc GTTGATAAGAAAGGAAAAATAATAGAT | pfk1 F2 (+) |
P4 | cccaagcttATGTACAAATCGATGTTTTGTATA | pfk1 F2 (-) |
P5 | ccgggaattc atTTTAATAATCATGATCATCAGAATC | pfk1 F3 (+) |
P6 | cccaagctt TAAACATTCTTGATCATCTTTTTCC | pfk1 F3 (-) |
P7 | ccgggaattc tcAAAAAAGGACAAAACCATCAACTT | pfk1 F4 (+) |
P8 | cccaagctt CATATTTTTTTCCATATCTTTAATG | pfk1 F4 (-) |
P9 | cgcggatcc gATGAAAAGCGGATTATTTTCTATG | pfk2 full length (+) |
P10 | ccggaattc TCACAATATATAAACTATATCATCGAATC | pfk2 full length (-) |
P11 | ccgggaattc ATAATGATAATGATAATAATAATATTATA | pfk2 F3 (+) |
P12 | cccaagctt tTTTAATTCCTTTTTATTATCAATTGT | pfk2 F3 (-) |
P13 | ccgggaattc GAATATCAAGAATATTTACCAAAAAC | pfk2 F4 (+) |
P14 | cccaagctt ATTATGAATATGAATATTTGTATTTGA | pfk2 F4 (-) |
P15 | aaactgcag TGAAAACAGAATGCGATTAGTTCG | pfk1 KO (+) |
P16 | cgcggatcc GATGATGCAAATGTTAATGGTAC | pfk1 KO (-) |
P17 | aaactgcag TTCTTATCTTATTGAACGGATCTC | pfk2 KO (+) |
P18 | cgcggatcc TTGTATGGTATATATAGTAGATGC | pfk2 KO (-) |
P19 | GATAGCGATTTTTTTTACTGTCTG | hrp2 3'UTR (+) |
P20 | GAAATTAACCCTCACTAAAGGG | plasmid backbone |
P21 | aaggaaaaaagcggccgc AATGAATAATGATAATATTGGGAG | pfk1 (+) |
P22 | cccaagctt AGATATAATACCATCCGTTTGG | pfk1 (-) |
P23 | ccggaattc AATGAAAAGCGGATTATTTTCTATG | pfk2 (+) |
P24 | cgcggatcc TTGTATGGTATATATAGTAGATGC | pfk2 (-) |
P25 | TTGTTGTGGTTCTACATCTCC | pfk2 (-) |
P26 | CACGAAGCCGCCACACATTGCC | hrp2 orf1 |
P27 | TTTGATCTTGTTCACTATGGC | pfk1 (-) |
P28 | cccaagctt ATTACAAAAATAATAATTGAAATTACT | pfk2 (-) |
P29 | GTAATACGACTCACTATAGGGC | plasmid backbone |
P30 | TTGCAATTCTGCTTCAGTTGG | cam (-) |