Figure 4

Recombinant PfTRAP undergoes phosphorylation in the presence of HepG2 and HEK 293-cell lysates. Recombinant PfTRAP fragments were incubated with HepG2 cell extract (a) and HEK 293 cell lysate (b) in the presence of P³² [γ ATP]. PfTRAP fragments were immunoprecipitated using anti-PfTRAP antibody and phosphorylation status was checked through autoradiography. (a) Lanes 1, HepG2 cell lysate; 2, A domain+HepG2 cell lysate; 3, A+RII+HepG2 cell lysate; 4, ECD+HepG2 cell lysate. ECD phosphorylation was also checked using recombinant MAPK (lane 6). MAPK was used as a control (lane 5). (b) Lanes1, HEK 293 cell lysate; 2, A+RII domain+ 293 cell lysate; 3, A+RII+RGD+ 293 cell lysate; 4, ECD+ 293 cell lysate.