Sequence | Target gene | |||
---|---|---|---|---|
Primers | Genus specific | Forward | 5'-GTTTAAGGCAACAACAGGT-3' | 18S rRNA |
Reverse | 5'-CAATAATCTATCCCCATCACGA-3' | |||
P. falciparum specific | Forward | 5-TTACATCAGGAATGTTATTGC-3 | cox1 | |
Reverse | 5-ATATTGGATCTCCTGCAAAT-3 | |||
Probes | Sensor | 5'-ACTCGTTATACATATCAGTGTAGCACGC (FLU)-3' | 18S rRNA | |
5'-(Red 705) | ||||
Anchor | GCAGCCTAGTTCATCTAAGGACATCACAG (P)- 3' |