Figure 1
From: Interactions between dendritic cells and CD4+ T cells during Plasmodium infection
Plasmodium yoelii -infected erythrocytes inhibit MHC-I but not MHC-II antigen presentation. (A-D) DCs differentiated in vitro were incubated alone (DC), with uninfected (DC+RBC), P. yoelii-infected erythrocytes (DC+iRBC) for 24 h before addition or not of purified OVA (5 mg/ml; A,B) or LACK (50 μg/ml; C,D) proteins for 5 h, followed by stimulation with LPS to increase antigen presentation. Cells were stained with antibodies recognizing (A,B) OVA epitope 257–264 bound to H-2b(antibody 25-D1) or (C,D) LACK epitope 156–173 bound to I-Ad (antibody 2C44). (A,C) Representative examples of FACs histograms of DCs incubated with protein OVA (A) or LACK (C) proteins (thick lines) or control DCs (thin lines). (B,D) Results are expressed as the difference in mean fluorescent intensity (MFI) of protein incubated DCs minus control DCs in triplicated determinations. *, significant difference (P < 0.01) in the intensity compared with DCs incubated alone.