Southern Blot Analysis of the pbmrp Gene. (A) Diagrammatic representation of the pbmrp locus organization. Black arrows above the locus represent the restriction sites within the gene or in flanking areas. Below the coding region, the black lines represent the 5' and 3' specific probes used. Genomic DNA from N clone and RC line was digested with Hind III (H3), Eco RI (RI), and Eco RV (RV). The predicted bands and their sizes are depicted by black arrows. (B) Both probes hybridize to a single band for each of the enzyme used: 14.1 kb for RV, 3.5 kb for RI, and 2.0 kb for H3 (left panel) and 9.5 kb with RI, 6.5 kb with H3, and approximately 2.5 kb with RV (right panel), respectively.