Figure 6

Effect of DB75 on peak expression time and intensity. Expression profiles for six developmentally expressed genes were compared in synchronized rings with exposure to 100 nM (grey) or 500 nM (black) DB75 or left untreated (white). (a) Time of invasion was approximated based on assessment of morphology, and peak expression post-invasion was estimated for all genes. (b) Expression intensity at time of peak expression was measured as the fold change in expression relative to the constitutively expressed 18s rRNA for exposed rings. Genes evaluated include: trophozoite antigen r45-like (Troph Ag); lactate dehydrogenase (ldh), isocitrate dehydrogenase (Iso);DNA primase (DNAp), Merozoite surface protein-1 (MSP-1); Merozoite surface protein-7(MSP-7).