Serum Anti- An. darlingi SGS levels in patients with different clinical spectrum of P. vivax infection. Serums were collected from volunteers with asymptomatic P. vivax infection (n = 50) and from patients with symptomatic infection (n = 74). An ELISA test was performed to assess the IgG anti-An. darlingi SGS. Box plot graph, with lines of the boxes representing 75th percentile, median and 25th percentile of the individual average OD values; whiskers represent the maximum and minimum values. Differences between groups were tested using Mann-Whitney test; p value is plotted.