Figure 1From: Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastorisDBL sequence alignment. The six DBL domains in VAR2CSA are compared to erythrocyte binding proteins. Cysteine residues are highlighted in blue shading and numbered -1 to 12 based on conservation between erythrocyte binding proteins. The end of the sequence homology between PfEMP1 and erythrocyte binding-type DBL domains is indicated with an arrowhead and grey shading of the final F/Y residue. The S1, S2 and S3 subdomains boundaries are indicated by arrows. The start and end residues in the original VAR2CSA DBL constructs are indicated by grey shading. There is a 26 amino acid spacer before the CXC motif in the DBL2 domain. Accession numbers are IT4-VAR2CSA (AAQ73926), EBA-175 (MAL7P1.176), and P. knowlesi α (M90466).Back to article page