Table 1 Expression of PfEMP1 domains in P. pastoris
From: Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris
Gene | Domain | Parasite strain | construct | MW1 | Protein Secreted | IE surface reactivity2 |
|---|---|---|---|---|---|---|
var2CSA | DBL1 | IT/FCR3 3 | H58 – S383 | 38.9 | ++ | yes |
var2CSA | DBL1 | 3D7 | H58 – I433 | 45.2 | ++ | yes |
var2CSA | DBL1 | 7G8 | H57-I437 | 46.3 | ++ | yes |
var2CSA | DBL1 | Dd2 | H57-I433 | 45.6 | ++ | yes |
var2CSA | ID1-DBL2 | IT/FCR3 | V490 – D883 | 44.5 | + | nt |
var2CSA | DBL2 | IT/FCR3 3 | K543 – K838 | 36.1 | - | nt |
var2CSA | DBL2 | IT/FCR3 | D517 – D883 | 41.6 | + | nt |
var2CSA | DBL2 | IT/FCR3 | D517 – L1024 | 58.4 | - | nt |
var2CSA | DBL2 | IT/FCR3 | G550 – L1024 | 54.6 | - | nt |
var2CSA | DBL2 | IT/FCR3 | D545 – L1024 | 55.2 | - | nt |
var2CSA | DBL2-ID2 | IT/FCR3 | D517 – G1229 | 81.6 | - | nt |
var2CSA | ID1-DBL2-ID2 | IT/FCR3 | V490 – G1229 | 84.4 | - | nt |
var2CSA | ID2 | IT/FCR3 | V878 – K1201 | 38.4 | - | nt |
var2CSA | ID2 | IT/FCR3 | S843 – G1229 | 45.2 | - | nt |
var2CSA | ID2 | IT/FCR3 | L1024 – G 1229 | 24.9 | - | nt |
var2CSA | ID2-DBL3 | IT/FCR3 | N 859 – C 1576 | 83.0 | - | nt |
var2CSA | DBL3 | IT/FCR3 3 | L1221 – E1541 | 37.3 | negligible | yes |
var2CSA | DBL3 | IT/FCR3 | C1202 – C1576 | 43.8 | + | yes |
var2CSA | DBL3 | 3D7 | C1224 – Y1510 | 33.2 | - | nt |
var2CSA | DBL3 | Dd2 | C1219 – Y1529 | 44.2 | - | nt |
var2CSA | DBL3 | Pc49 | C1227 – Y1540 | 43.8 | - | nt |
var2CSA | DBL3 | 7G8 | C1199 – E1587 | 45.0 | ++ | nt |
var2CSA | DBL4 | IT/FCR3 3 | S1594 – V1888 | 34.7 | ++ | no4 |
var2CSA | DBL4-DBL5 | IT/FCR3 | G1588 – E2288 | 85.4 | - | nt |
var2CSA | DBL4-DBL5 | IT/FCR3 | S1594 – E2288 | 82.5 | - | nt |
var2CSA | DBL4-ID4-DBL5 | IT/FCR3 | S1594 – R2271 | 66.8 | - | nt |
var2CSA | DBL4-DBL5 | 3D7 | S1576 – T2291 | 85.6 | - | nt |
var2CSA | DBL4-DBL5 | 3D7 | G1550 – T2291 | 88.3 | - | nt |
var2CSA | DBL4-DBL5 | 7G8 | S1604 – T2318 | 89.2 | - | nt |
var2CSA | DBL4-DBL5 | 7G8 | G1578 – T2318 | 88.3 | + | nt |
var2CSA | DBL5 | IT/FCR3 3 | L2003 – L2270 | 31.7 | - | nt |
var2CSA | DBL5 | IT/FCR3 | N1893 – N2290 | 46.6 | - | nt |
var2CSA | DBL5 | IT/FCR3 | K1984 – E2288 | 36.6 | + | yes |
var2CSA | DBL5 | IT/FCR3 | E1971 – N2290 | 37.8 | - | nt |
var2CSA | DBL5 | 7G8 | Q2000 – T2318 | 38.8 | ++ | yes |
var2CSA | DBL5 | 3D7 | L1997 – A2269 | 32.2 | - | nt |
var2CSA | DBL5 | 3D7 | K1978 – T2291 | 37.9 | - | nt |
var2CSA | DBL5 | 3D7 | L1997 – T2291 | 35.8 | - | nt |
var2CSA | DBL5 | 3D7 | E1964 – T2291 | 39.6 | - | nt |
var2CSA | DBL5 | 3D7 | K1923 – T2291 | 44.6 | - | nt |
var2CSA | DBL5 | 3D7 | N1888 – T2291 | 48.3 | + | yes |
var2CSA | DBL6 | IT/FCR3 3 | E2322 – E2590 | 32.7 | ++ | yes |