Table 1 The primers and annealing temperatures used for PCR to amplify and sequence the exons of the G6PD gene
Exons | PCR product size (bp) | PCR anealing temperature (°C) | Primers |
|---|---|---|---|
1A | 305 | 62 | F 5'-CTTGAACCCGCGAACAGGCGA |
| Â | Â | Â | R 5'-TCTCGGGCACCTGCGCTGGA |
1B | 365 | 68 | F 5'-GCAGAGCAGCGGCAGCGGGTAT |
| Â | Â | Â | R 5'-TATTTTACCGCCGCGCGGCGCA |
2 | 241 | 65 | F 5'-CTCAAGAAAGGGGCTAACTTCTCAA |
| Â | Â | Â | R 5'-GCACTTCCTGGCTTTTAAGATTGGG |
3+4 | 352 | 60 | F 5'-CAGGCCAACTTCTAACCACACACCT |
| Â | Â | Â | R 5'-CCGAAGCTGGCCATGCTGGG |
5 | 295 | 65 | F 5'-CTGTCTGTGTGTCTGTCTGTCC |
| Â | Â | Â | R 5'-GGCCAGCCTGGCAGGCGGGAAGG |
6 | 264 | 58 | F 5'-ACTCCCCGAAGAGGGGTTCAAGG |
| Â | Â | Â | R 5'-GAGGCTCCTGAGTACCACCC |
7 | 234 | 65 | F 5'-CAAGGTCAGTTCCTCCACCTTGCC |
| Â | Â | Â | R 5'-GAAGAGTAGCCCTGCAGGGTGACT |
8 | 164 | 72 | F 5'-GGAGCTAAGGCGAGCTCTGGC |
| Â | Â | Â | R 5'-GGCATGCTCCTGGGGACTGGG |
9 | 253 | 72 | F 5'-CAAGGAGCCCATTCTCTCCCTT |
| Â | Â | Â | R 5'-TGCCTTGCTGGGCCTCGAAGG |
10 | 318 | 72 | F 5'-CTGAGAGAGCTGGTGCTGAGG |
| Â | Â | Â | R 5'-AGGCCGCCCACCCTCCACACT |
11 | 160 | 65 | F 5'-GCAGGCAGTGGCATCAGCAAG |
| Â | Â | Â | R 5'-CCCCATAGCCCACAGGTATGCAGG |
12+13A | 368 | 72 | F 5'-TGTGTGCCACCGGCCTCCCA |
| Â | Â | Â | R 5'-GGGAAGGAGGGTGGCCGTGG |
13B | 364 | 68 | F 5'-GTGGGTGAACCCCCACAAGGTC |
| Â | Â | Â | R 5'-TGGCCCCACTCAGGAGTGAGAC |
13C | 377 | 68 | F 5'-CCATTCGTCTGTCCCAGAGCTTA |
| Â | Â | Â | R 5'-TGGGACAAGGAAGTGGGTCCTCA |