Figure 2

Ingenuity Pathway network analysis. Transcripts altered ≥ 1.8-fold (p ≤ 0.025) in 15-HETE-treated RAW 264.7 cells (i.e., focus genes) were overlaid onto a global molecular network developed from information contained in the Ingenuity knowledge base (IKB). Networks of these focus genes were algorithmically generated based on their connectivity. Networks show direct interactions between focus genes altered by 15-HETE treatment and associated molecules within the IKB. Genes or gene products are represented as nodes, and the biological relationship between two nodes is represented as an edge (line). White nodes represent IKB molecules that are associated with focus genes. All edges are supported by at least one reference stored in the IKB. The intensity of node color indicates the degree of up- (red) or down- (green) regulation. Networks reflect (A) lipid metabolism and small molecule biochemistry and (B) molecular transport and cellular movement.