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Figure 1 | Malaria Journal

Figure 1

From: FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance

Figure 1

Schematic representation of the FlexiChip analysis method. A) SNPs are detected by Single Base Extension (SBE) using Sequenase and ddNTP labelled with Cyanine 3 or 5 using a specific probe that hybridizes one nucleotide upstream of the SNP site. B) Products of the SBE reaction are hybridized on FlexiChip by their Zip-code oligonucleotides. After washing and drying, the slides are scanned at two wave lengths. C) Analysis algorithm is based on a mixture model and allows accurate SNP identification. The results are stored in Excel file.

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Malaria Journal

ISSN: 1475-2875

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