The malaria candidate vaccine liver stage antigen-3 is highly conserved in Plasmodium falciparum isolates from diverse geographical areas

Table 1 Primer pairs used to amplify the lsa-3 gene in clinical isolates genomic material.

PCR fragment	Primer forward (5'→3')	Primer reverse (5'→3')	PCR length ^a
5T	GTTAGAAAATGACAAATAGTAATTAC	GTGTTGTTGTTCTTGTTGAACAC	829
Repeats I/II (PCR1)	GTAAAAAGTGTTCAACAAGAAC	TTCCTCAGTTTCGATACCACC	1188
Repeats I/II (PCR2)	GAAAATATTTTGGAGGAAAGTCAAG	ACTGTCCTTTATTTCCTCAGTTTCG	1251
6.1-6.2	GGTATCGAAACTGAGGAAATAAAGG	CATAGCAGGAACATCAACATCCAC	789
C1-C2	GAAACTGTAACTGAACATGTAGAAC	CTTCAAGATCTTTTAAATCAGATAC	745
Repeats III	GATGTCGAAGAAGACAAGATCG	CTTTTCAATGCGTTTCTCTTTTTGG	855
3T	GGTGAAGACAAAGATGAAGTTATAG	GCAATTTTTTATTTTGATTTTTGCGTTC	665

^a, PCR fragment length in base pairs according to the lsa-3 nucleotide sequence in P. falciparum strain 3D7.

ISSN: 1475-2875