Fluorescence units (AU) | ||||
---|---|---|---|---|
SYBRGreen I® | PicoGreen® | |||
% parasitaemia | IRBC | IRBC | IRBC-WBC | IRBC-WBC/W |
5 | 15 ± 3 | 229 ± 12 | 243 ± 4 | 279 ± 8 |
2.5 | 9 ± 2 | 127 ± 7 | 141 ± 8 | 161 ± 7 |
1.3 | 6 ± 2 | 52 ± 1 | 83 ± 9 | 80 ± 4 |
0.6 | 5 ± 4 | 36 ± 3 | 36 ± 11 | 45 ± 2 |
0.3 | ND1 | 20 ± 5 | 20 ± 8 | 31 ± 4 |
0.1 | ND | 11 ± 3 | 11 ± 3 | 14 ± 2 |
0.05 | ND | 5 ± 4 | ND | 7 ± 3 |
Limits of detection and quantification (parasitaemia %) | ||||
LOD | 1.37 | 0.23 | 0.21 | 0.24 |
LOQ | 4.14 | 0.71 | 0.74 | 0.64 |