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Table 1 Fluorescence of PG and SGI - DNA adducts on Plasmodium infected erythrocytes at low parasitaemia after removal of haemoglobin and detergents

From: Haemoglobin interference and increased sensitivity of fluorimetric assays for quantification of low-parasitaemia Plasmodium infected erythrocytes

  Fluorescence units (AU)
  SYBRGreen I® PicoGreen®
% parasitaemia IRBC IRBC IRBC-WBC IRBC-WBC/W
5 15 ± 3 229 ± 12 243 ± 4 279 ± 8
2.5 9 ± 2 127 ± 7 141 ± 8 161 ± 7
1.3 6 ± 2 52 ± 1 83 ± 9 80 ± 4
0.6 5 ± 4 36 ± 3 36 ± 11 45 ± 2
0.3 ND1 20 ± 5 20 ± 8 31 ± 4
0.1 ND 11 ± 3 11 ± 3 14 ± 2
0.05 ND 5 ± 4 ND 7 ± 3
Limits of detection and quantification (parasitaemia %)
LOD 1.37 0.23 0.21 0.24
LOQ 4.14 0.71 0.74 0.64
  1. IRBC = Infected red-blood cells; IRBC-WBC = Infected red-blood cells whithout white blood cells removal; IRBC-WBC/W = Infected red-blood cells after white blood cells removal; ND = not detected.