Table 1 Primers used to amplify target sequences in cytochromes P450 isoenzymes and N-acetyltransferase 2 genes, MgCl₂ [μl] for the master mix and annealing temperature [°C] used to amplify target sequence in cytochromes P450 isoenzyme genes and N-acetyltransferase 2 genes.

CYP2A6*2 (479T>A, L160H)

forward

reverse

5'-TCTCTCTCTCTACCTCGACAT-3'

5'-GTTCCTCGTCCTGGGTGTT-3'

3

64

CYP2B6*5 (1459C>T, R487C)

forward

reverse

5'-CCAGAAGACATCGATCTGAC-3'

5'-TCTCTCAGAGGCAGGAAGTT-3'

2

64

CYP2B6*6 (516G>T, Q172H)

forward

reverse

5'-TGAGTGATGGCAGACAATCACA-3'

5'-CAAGTTGAGCATCTTCAGGAACT-3'

2

64

CYP2C8*3 (416G>A, R139K)

forward

reverse

5'-CTAAAGGACTTGGTAGGTGCA-3'

5'-CAGGATGCGCAATGAAGACC-3'

2

64

CYP2C9*3/*5 (1075A>C, I359L/1080C>G, D360E)

forward

reverse

5'-CTGGTTTATGGCAGTTACACATT-3'

5'-GAGAAAGTCCAGTTAAACTGCC-3'

2

64

CYP2C19*3 (636G>A, W212X)

forward

reverse

5'-GGGAATTCATAGGTAAGATATTA-3'

5'-GGAGTGATATAAGCACGCTTTG-3'

2

64

CYP2D6*4 (1846G>A, splicing defect)

forward

reverse

5'-CCGCCTTCGCCAACCACT-3'

5'-CCCTGCAGAGACTCCTCGGT-3'

2

64

CYP2D6*10 (100C>T, P34S)

forward

reverse

5'-CCCATTTGGTAGTGAGGCAGGT-3'

5'-CCCCTTCTCAGCCTGGCTTCTTG-3'

2

64

CYP2D6*10 (4180G>C, S486T)

forward

reverse

5'-AGCCACCATGGTGTCTTTGCT-3'

5'-TTGCCCTGAGGAGGATGATC-3'

2

64

CYP2D6*17 (1023C>T, T107I)

forward

reverse

5'-CGCGAGGCGCTGGTGACCAA-3'

5'-CCAGCTCGGACTACGGTCATCAC-3'

2

64

CYP2D6*17 (2850C>T, R296C)

forward

reverse

5'-GACTCTGTACCTCCTATCCACGTCA-3'

5'-TCCCTCGGCCCCTGCACTGTTT-3'

2

64

CYP3A4*1B (-392A>G)

forward

reverse

5'-CTCACCTCTGTTCAGGGAAAC-3'

5'-ATGGCCAAGTCTGGGATGAG-3'

2

64

CYP3A5*3 (6986A>G, splicing defect)

forward

reverse

5'-TGGAGAGTGGCATAGGAGATAC-3'

5'-CCATACCCCTAGTTGTACGACACA-3'

2.5

64

NAT2*5/*6/*7/*14 (341T>C, I114T/590G>A, R197Q/857G>A, G286E/191G>A, R64Q)

forward

reverse

5'-GGGATCATGGACATTGAAGCATATT-3'

5'-ACGTGAGGGTAGAGAGGATATCTG-3'

2

64