Figure 5
Immunogenicity of a virosomal FB-12 formulation produced according to the rules of GMP. Development of anti-peptide IgG responses in mice (A) and in rabbits (B). Shown are serial dilutions of sera in peptide-ELISA (means +/-SD). Cross-reactivity of anti-FB-12 IgG responses of mice (C) and rabbits (D) with cultured P. falciparum blood-stage parasites in IFA. E: Western-blot analysis of mouse serum samples with blood stage parasite lysate. Pooled pre-immune serum (E, lane 1) and individual serum samples (E, lanes 2-6) collected two weeks after the second immunization were used at a dilution of 1:10'000. F: Mean avidity indices for anti-FB-12 IgG responses of rabbits three weeks after the first, second and third immunization. The avidity index corresponds to the NH4SCN concentration (M) where 50% of the bound antibodies are eluted. Shown are means +/-SD. Wilcoxon signed-rank test was used to calculate the statistical significance of a difference in avidity between the third and the first vaccination.