Figure 1

An anti-mouse GIMAP1 monoclonal antibody was produced that is specific and can detect endogenous mouse GIMAP1. A. N-terminally myc-tagged GIMAP constructs were transiently transfected into HEK293T cells. The lysates were Western blotted with either an anti-myc antibody 9E10 (upper panel) or the anti-GIMAP1 mAb MAC420 (lower panel). B. The domain structure of mouse GIMAP1 and the two truncates used in A. G1-G5 are the GIMAP versions of the canonical GTP binding motifs, TM indicates the putative transmembrane domain. C. Tissues were removed from a C57BL/6 mouse, homogenised in lysis buffer and 100 μg of protein from each sample was Western blotted with the anti-GIMAP1 mAb (MAC420) or anti-β-actin (AC-15). Estimated molecular weights are in kDa.