Splenic and hepatic particle trapping during P. chabaudi malaria. (A) Localization of 3 μm green fluorescent beads in splenic and hepatic cryosections of non-vaccinated BALB/c mice. The scale bar represents 200 μm. (B) Quantification of splenic and hepatic trapping. BALB/c mice were vaccinated (+vacc) or not vaccinated (-vacc) and then were challenged with 106 pRBC. Parasitaemia, spleen weight, and total number of beads per spleen, and numbers of beads per 100 mg spleen or liver are given as means ± S.E.M. The crisis phase (days 8–13 p.i.) is accentuated in grey. ***, p < 0.001 vs. non-vaccinated control on the same day p.i. *, p < 0.05 vs. non-vaccinated control on the same day p.i.