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Table 3 Msp1 and msp2 genotyping by CE and gel electrophoresis of 240 microscopy P. falciparum- positive samples from asymptomatic children

From: Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)

  Capillary electrophoresis Gel electrophoresis
  msp1 msp2 msp1 msp2
  K1 MAD20 RO33 total FC27 IC total K1 MAD20 RO33 total FC27 IC total
PCR positive 195 129 124 223 169 204 223 197 129 126 223 166 201 223
Fragmentsa 314 160 124 598 230 415 645 225 147 126 498 191 253 444
Frequencyb 33 14 1 48 20 76 96 n.d. n.d. n.d. n.d. n.d. n.d. n.d.
Meanc
(95%CI)
1.61 1.24 1.0 2.68
(2.50–2.86)
1.37 2.03 2.89
(2.66–3.13)
1.14 1.14 1.0 2.23
(2.11–2.36)
1.15 1.26 1.99
(1.89–2.09)
  1. a Total number of detected msp1 and msp2 fragments
  2. b number of different alleles detected in all samples, size bins of 3 bp
  3. c Number of alleles/sample, excluding all parasite-negative samples
  4. n.d. not determined