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Table 3 Msp1 and msp2 genotyping by CE and gel electrophoresis of 240 microscopy P. falciparum- positive samples from asymptomatic children

From: Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)

 

Capillary electrophoresis

Gel electrophoresis

 

msp1

msp2

msp1

msp2

 

K1

MAD20

RO33

total

FC27

IC

total

K1

MAD20

RO33

total

FC27

IC

total

PCR positive

195

129

124

223

169

204

223

197

129

126

223

166

201

223

Fragmentsa

314

160

124

598

230

415

645

225

147

126

498

191

253

444

Frequencyb

33

14

1

48

20

76

96

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

Meanc

(95%CI)

1.61

1.24

1.0

2.68

(2.50–2.86)

1.37

2.03

2.89

(2.66–3.13)

1.14

1.14

1.0

2.23

(2.11–2.36)

1.15

1.26

1.99

(1.89–2.09)

  1. a Total number of detected msp1 and msp2 fragments
  2. b number of different alleles detected in all samples, size bins of 3 bp
  3. c Number of alleles/sample, excluding all parasite-negative samples
  4. n.d. not determined