Figure 3

FRET/MCA for detection of kdr mutations in An. epiroticus and An. minimus s.l. The FRET/MCA of the DIIS6 region of the para-type sodium channel gene plotted as the first negative derivative of the relative fluorescence unit (-d(RFU)/dT) versus temperature function. In all panels, the thicker blue horizontal line denotes the threshold for background fluorescence, and the curve entirely below denotes the results for the non-template control (gray). (A) FRET/MCA I An. epiroticus plasmids, from low to high Tm: the L1014S (green), the L1014F (purple) and wild type L1014 plasmid (blue). Melting peaks were identified at 60.3°C, 61.8°C and 64.5°C, respectively. (B) FRET/MCA II An. minimus s.s. plasmids, from low to high Tm: the L1014S plasmid (green), Tm of 59.7°C; the L1014F plasmid (purple), Tm of 60.9°C; and the wild type L1014 plasmid (blue), Tm of 63.9°C. (C) FRET/MCA II from low to high Tm: the L1014S plasmid (green), Tm of 59.7°C; the An. harrisoni mosquito of VLSB with a different melting peak with Tm of 62.1°C (pink); and wild type An. harrisoni specimens (blue), Tm of 63.9°C.