Figure 4

Sequences of the DIIS6 region of the para -type sodium channel gene obtained for An. harrisoni mosquitoes with respectively a Tm of 63.9°C (wild type, WT) and 62.1°C. Sequences of the 132 bp fragment of theDIIS6 region of the para-type sodium channel gene obtained after amplification with primers MinF-ROX and FunminR of An. harrisoni mosquitoes with respectively a Tm of 63.9°C (wild type, WT) [GenBank: EU155387] and a Tm of 62.1°C (VSLB) [GenBank: EU258605] in the FRET/MCA. The triplet TTA at codon 1014 is given in bold, while the hybridization place of the probe is underlined. A line (-) indicates a nucleotide identity compared to the wild type An. harrisoni sequence. R = heterozygote A/G