Table 1 Primer and Probe sequences used in real--time quantitative PCR
From: Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice
Gene | Primer/probe |
|---|---|
18s rRNA | 5'-CGATGTGTGTCTAACACAAGGAAGT-3'F |
| Â | 5'-CATAGGCTTTAACACCTAAGCACAG-3'R |
| Â | 5'-FAM-TATGTAAAACGAGTGTTAAAT-MGB-3' |
2-Cys Prx | 5'-AAACACCATTGTCACAAGGAGGTA-3'F |
| Â | 5'-ACAAACGCTCTTAATGCTACATTTC-3'R |
| Â | 5'-FAM-AAGCATACTTTGATATCCG-MGB-3' |
g-GCS | 5'-AATGAGTATGTGCTGCCAACAAAT-3'F |
| Â | 5'-AAGAATCCACCTAAATATGGTGTACATG-3'R |
| Â | 5'-FAM-AGCTAGCTGTAATTGC-MGB-3' |
Grx | 5'-GGGAAAAGTTCCGTACCAAGAAT-3'F |
| Â | 5'-TTCCAATGTCTGGAGTCTCTCTGA-3'R |
| Â | 5'-FAM-ACCAGTCATCACATCCGCCGACATT-MGB-3' |
TrxR | 5'-AGTCACTCAAGGAATGGGATTGG-3'F |
| Â | 5'-TGCATAAGACAAACCCGATGATAA-3' |
| Â | 5'-FAM-TTCATCCAACAGATGCAG-MGB-3' |