Figure 3

Confocal microscopy of Gal4p fragments in P. falciparum. Two truncated forms of Gal4-GFP were constructed and labeled as pGal4(1-74)-GFP and pGal4(75-147)-GFP. Bright field, DAPI, GFP and DAPI merged with GFP images were taken by a laser-scanning confocal microscope from late-stage parasites. The first 74 residues of Gal4p were sufficient for transporting GFP into the nucleus as shown by confocal microscopy.