The mean gametocyte circulation time based on Pfs25 QT-NASBA gametocyte density after non-ACT and ACT. Log gametocyte density/μL is given on the Y-axis, the day of follow up after initiation of treatment. Symbols and error bars indicate the field data with 95% confidence interval, lines fitted values. The dotted line indicates the lower threshold for gametocyte detection by QT-NASBA, 0.02 gametocytes/μL. The trial was conducted in Kenya; treatment regimens were non-ACT (open diamonds; SP+AQ administered on day 0-2) and ACT (closed triangles; SP+AS or AL administered on day 0-2). The estimated mean circulation time of gametocytes in this trial was 6.53 days (95% CI 4.84-8.80) after non-ACT treatment and 5.04 days (95% CI 4.20-6.06) after ACT treatment, based on data between d3 and d28.