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Table 1 Genotyping results of An. culicifacies samples by PCR-based assays and allelic association between L1014 and V1010 mutations as revealed by DNA sequencing

From: Presence of two alternative kdr-like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na+ channel of Anopheles culicifacies from Orissa, India

   L1014
Genotyping
methods 		Genotypes         
    L/L L/F L/S F/S F/F S/S Total
PCR-based assays    176 (0.786) 26 (0.116) 18 (0.080) 2 (0.009) 2 (0.009) 0 (0.000) 224
   V/V 64 26 0 0 2 0 92
   V/L 0 0 17* 2 0 0 19*
DNA-sequencing V1010         
   L/L 0 0 0 0 0 0 0
   Total 64 26 17 2 2 0 111
  1. Figures in parenthesis indicate genotype frequencies. Letters L, F, S and V refer to leucine, phenylalanine, serine and valine amino acids, respectively.
  2. *In two samples V1010L substitution was due to 3028G>C transversion and in rest samples due to 3028G>T transversion.
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Malaria Journal

ISSN: 1475-2875

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