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Table 1 Genotyping results of An. culicifacies samples by PCR-based assays and allelic association between L1014 and V1010 mutations as revealed by DNA sequencing

From: Presence of two alternative kdr-like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na+ channel of Anopheles culicifacies from Orissa, India

  

L1014

Genotyping

methods

Genotypes

        
   

L/L

L/F

L/S

F/S

F/F

S/S

Total

PCR-based assays

  

176 (0.786)

26 (0.116)

18 (0.080)

2 (0.009)

2 (0.009)

0 (0.000)

224

  

V/V

64

26

0

0

2

0

92

  

V/L

0

0

17*

2

0

0

19*

DNA-sequencing

V1010

        
  

L/L

0

0

0

0

0

0

0

  

Total

64

26

17

2

2

0

111

  1. Figures in parenthesis indicate genotype frequencies. Letters L, F, S and V refer to leucine, phenylalanine, serine and valine amino acids, respectively.
  2. *In two samples V1010L substitution was due to 3028G>C transversion and in rest samples due to 3028G>T transversion.