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Figure 1 | Malaria Journal

Figure 1

From: Cloning, expression and transmission-blocking activity of anti-PvWARP, malaria vaccine candidate, in Anopheles stephensi mysorensis

Figure 1

Cloning, expression and characterization of PvWARP. A) Digestion result of the extracted plasmid (pET-23a) from DH5a by using BamH I and Hind III restriction enzymes on 1.5% agarose gel. M: DNA molecular marker. B) Optimization of PvWARP expression in pET-23a at E. coli strain BL21 host in 4, 6 and 24 hours after induction. B: before induction, M: protein molecular weight maker (kDa). C) Western blotting of polyclonal antibody for PvWARP. Purified protein was blotted on nitro cellulose membrane and stained by diaminobenzidine; lanes 1: negative control (mice without any protein injection), lane 3 mice immunized with Freund's Adjuvant lane 5, protein size marker; lanes 2, 4 and 6: result of Western Blot in mice immunized with rPvWARP.

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Malaria Journal

ISSN: 1475-2875

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