Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection

Table 2 Genotype abundance for uninfected and P. falciparum-infected molecular form A. gambiae associated with P. falciparum infection status.

Chromosome	Gene Location	Locus	Genotype	Mutation Type	^aM		^bS1		^cS2
					^dUI	^eI	UI	I	UI	I
3L	7,103,451-7,105,904	Ins34*	CC	synonymous	17	6	20	19	25	18
			CT	synonymous	8	19	23	33	10	18
			TT	synonymous	5	0	7	6	3	1
3L	8,174,795-8,178,377	Toll5B1* ¹	CC	synonymous	1	18	6	47	29	35
			TC	synonymous	29	7	43	11	9	2
			TT	synonymous	0	0	1	0	0	0
3L	8,174,795-8,178,377	Toll5B6*	AA	nonsynonymous	2	4	4	4	0	5
			AG	nonsynonymous	5	2	18	3	13	12
			GG	nonsynonymous	23	19	28	51	25	20
3L	7,103,451-7,105,904	Ins35¹	CC	nonsynonymous	27	17	40	45	37	35
			CT	nonsynonymous	3	8	10	13	1	1
			TT	nonsynonymous	0	0	0	0	0	1
3L	7,103,451-7,105,904	Ins32	AA	synonymous	2	0	5	1	2	1
			AG	synonymous	9	16	25	29	11	17
			GG	synonymous	19	9	20	28	25	19
3L	7,103,451-7,105,904	Ins33	CC	nonsynonymous	24	14	29	30	1	1
			CT	nonsynonymous	5	11	20	25	10	9
			TT	nonsynonymous	1	0	1	3	27	27
2R	36,863,007-36,864,732	Mkk43	AA	synonymous	0	0	1	0	0	0
			AG	synonymous	5	7	10	3	1	4
			GG	synonymous	25	18	39	55	37	33
3L	8,174,795-8,178,377	Toll5B2	AA	nonsynonymous	30	25	46	56	37	36
			AC	nonsynonymous	0	0	4	2	1	1
			CC	nonsynonymous	0	0	0	0	0	0

Groups included M uninfected, M infected, S1 uninfected, S1 infected, S2 uninfected, S2 infected. ^aM forms collected in Doneguebougou, Selinkenyi, and Sidaribougou, ^bS forms collected in Bancomana, Doneguebougou, Selinkenyi and Sidaribougou, ^cS forms collected in Pimperena. ^dUninfected (UI); ^eInfected (I) with P. falciparum.
*Significant association with infection status (p < 0.0014), numbers of individuals in bold. ¹Significant difference in genotype frequencies between S1 and S2 (p < 0.0014).

ISSN: 1475-2875