DBL5ε conserved and immunogenic positions. A. Positive selection sites mapped in the DBL5ε structure model are colored in red. In orange are shown sites which correspond to differential positions associated to parity and birth weight in the alignment of the 172 sequences from pregnant women. In purple is shown the position that is both under positive selection pressure and correlated with birth weight and parity. B. Similar to A, but rotated 180 degrees. C. Epitopes identified previously, in blue are shown the conserved residues, and in green, the variable residues, predicted to be targeted by surface reactive antibodies.