Figure 3

Example of overlay culture from one monolayer. A monolayer made from a Plasmion-enriched culture (XP5) at 66.8% parasitaemia was overlaid with uninfected cells forming overlay#1 for 1 hour. This was removed and replaced with fresh uninfected red cells for a further hour (overlay#2), and then with a third replacement (overlay#3) and finally a fourth (overlay #4) for a final hour. There was no detectable parasitaemia in overlay #1. Peak parasitaemia was observed in overlay #3. Only ring stage parasites were seen in overlay #2 to #4.