Figure 1

PfA-M1 is expressed under the p120 form before the apparition of p96 and p68. The expression of the p120, p96, and p68 forms of PfA-M1 is stage specific. Soluble parasite extracts from: non infected erythrocytes (lane 1); infected erythrocytes 6-12 hours post invasion (lane 2); infected erythrocytes 18-24 hours post invasion (lane 3); infected erythrocytes 30-36 hours post invasion (lane 4); infected erythrocytes 38-44 hours post invasion (lane 5), were blotted with anti-MAP1 antibodies [6] to detect PfA-M1 (A) and anti-Pf Aldolase antibodies (B) [15] as control. The p120 is expressed early in the erythrocytic stages and it is detectable at 6-12 hours post invasion (lane 2). The amount of p120 increases to a maximum at 38-44 hours post invasion (lane 5). In contrast p68 is not detected 6-12 hours post invasion (lane 2) but is appearing 18-24 hours post invasion (lane 3). Traces of p96 are observed at 18-24 hours and 30-36 hours post invasion (lanes 3 and 4).