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Table 1 Characteristics of genotyping msp2 using agarose gel versus capillary electrophoresis.

From: Gel versus capillary electrophoresis genotyping for categorizing treatment outcomes in two anti-malarial trials in Uganda

Study Location Characteristic Gela CEb P valuec
Kanungu
(n = 90)
Probability two randomly selected alleles will match by chanced 0.066 0.025 <0.001
  MOI Day 0e, mean (SD) 2.73 (1.53) 3.82 (2.58) <0.001
  MOI Day Ff, mean (SD) 1.23 (1.14) 2.02 (1.82) <0.001
  Pmatch g, median (IQR) 0.20 (0.11 - 0.36) 0.16 (0.06 - 0.26) 0.001
Apac
(n = 209)
Probability two randomly selected alleles will match 0.061 0.028 <0.001
  MOI Day 0, mean (SD) 3.65 (1.80) 5.06 (3.42) <0.001
  MOI Day F, mean (SD) 2.42 (1.59) 3.26 (2.62) <0.001
  Pmatch, median (IQR) 0.41 (0.23 - 0.59) 0.27 (0.12 - 0.50) <0.001
  1. a Gel, Agarose gel electrophoresis
  2. b CE, Capillary electrophoresis
  3. c P values testing the hypothesis Gel is different than CE using a bootstrap test for the probability two alleles will match by chance, and the Wilcoxon Rank Sum test for other characteristics
  4. d Calculated using the empiric distribution of Day 0 alleles and criteria for a match based on the electrophoresis method
  5. e Multiplicity of infection (number of alleles detected) on Day 0 (day of anti-malarial treatment)
  6. f Multiplicity of infection on Day F (day of failure, i.e. recurrent parasitaemia)
  7. g Probability of a genotyping match occurring by chance for each sample, calculated using the actual alleles present on Day 0 and the multiplicity of infection on Day F (MOI-F). Ten thousand random combinations of MOI-F alleles were chosen for Day F using the empiric frequency distribution, and Pmatch is the proportion of these combinations that had at least one allele match with the Day 0 alleles.