Table 1 The nucleotide and amino acid substitutions at (a) dhfr and (b) dhps genes screened for by PCR-SSOP
dhfr | Â | Â | Â | Â | Â |
|---|---|---|---|---|---|
Codon | 50 | 51 | 59 | 108 | 164 |
Wild type | Cys (C) TGT | Asn (N) AAT | Cys (C) TGT | Ser (S) AGC | Ile (I) ATA |
| Â | Â | AAC | Â | Â | Â |
Mutant | Arg (R) | Ile (I) | Arg (R) | Asn (N) | Leu (L) |
| Â | CGT | ATT | CGT | AAC | TTA |
| Â | Â | Â | Â | Thr (T) | Â |
| Â | Â | Â | Â | ACC | Â |
dhps | Â | Â | Â | Â | Â |
Codon | 436 | 437 | 540 | 581 | 613 |
Wild type | Ser (S) | Ala (A) | Lys (K) | Ala (A) | Ala (A) |
| Â | TCT | GCT | AAA | GCG | GCC |
Mutant | Phe (F) | Gly (G) | Glu (E) | Gly (G) | Ser (S) |
| Â | TTT | GGT | GAA | GGG | TCC |
| Â | Ala (A) | Â | Â | Â | Thr (T) |
| Â | GCT | Â | Â | Â | ACC |
| Â | Cys (C) | Â | Â | Â | Â |
| Â | TGT | Â | Â | Â | Â |