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Table 4 ELISpot IFN-γ activity of AMA1 15-mer peptides after depletion of CD4+ and CD8+ T cells

From: Identification and localization of minimal MHC-restricted CD8+ T cell epitopes within the Plasmodium falciparum AMA1 protein

Vol. Pool Peptide number* Sequence Control depletion sfc/m CD4 depletion sfc/m (%) CD8 depletion sfc/m (%)
001 Ap1    155 222 (+43%) 55 (-65%)
   1 LLSAFEFTYMINFGR 85 58 (-32%) 37 (-56%)
   5 KEYEYPLHQEHTYQQ 72 168 (+133%) 17 (-76%)
   16 TSNNEVVVKEEYDE 103 252 (+148%) 13 (-88%)
002 Ap1    187 148 (-21%) 5 (-97%)
   3 INEHREHPKEYEYPL 185 152 (-18%) 11 (-94%)
005 Ap1    267 173 (-35%) 11 (-96%)
   3 INEHREHPKEYEYPL 233 145 (-37%) 0 (-100%)
  Ap4    273 117 (-57%) 0 (-100%)
   7 LMSPMTLDEMRHFYK 204 137 (-32%) 1 (-100%)
012 Ap10    48 37 (-23%) 0 (-100%)
   16 TSNNEVVVKEEYDE 79 90 (+14%) 0 (-100%)
  1. The percent change in ELISpot activity is shown after depletion of CD4+ or CD8+ T cells. Depletion of CD8+ T cells reduced ELISpot activity by 56-100%, whereas CD4+ T cell depletion reduced activity by no more than 57%. Although this suggested that the primary epitopes within these peptide pools and 15-mers are CD8+ T cell epitopes, some peptides may also contain CD4+ T cell epitopes. v001 and v012 showed an increase in ELISpot activity after CD4+ T cell depletion that may indicate the removal of regulatory T cells that had been suppressing CD8+ T cell activity.
  2. * See last column of Table 3.