ELISA showing reactivity of sera from P. vivax -infected patients against rPvTRAMP. Columns 1-20 correspond to recognition by sera from P. vivax malaria patients. Columns 21-23 showed recognition of rPvTRAMP by healthy individuals that had never had an episode of P. vivax malaria. rPvTRAMP used in this assay was resuspended in urea and thoroughly dialyzed against PBS for its refolding. Each column is shown with its corresponding standard deviation.