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Figure 5 | Malaria Journal

Figure 5

From: A protein-centric approach for the identification of folate enzymes from the malarial parasite, Plasmodium falciparum, using OFFGEL™ solution-based isoelectric focussing and mass spectrometry

Figure 5

Western blot analysis. A. SDS-PAGE gel of OFFGEL™ fractions probed with anti-DHFS-FPGS. B. SDS-PAGE gel of OFFGEL™ fractions probed with anti-GTPCI. Anti-PTPS was used as a positive control after blotting and probing for DHFS-FPGS. The isoelectric point and molecular mass of both DHFS-FPGS and GTPCI differ significantly from their theoretical values. DHFS-FPGS would be expected to be identified from lane 6 at molecular mass 60 kDa, yet western blotting analysis places it in lane 3. GTPCI has a theoretical mass of 46 kDa and would be expected to be identified from lane 12, yet this analysis identifies it as being found in lanes 9 and 10 and at mass 60 kDa

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Malaria Journal

ISSN: 1475-2875

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