Figure 4
Spatial variation in selection parameters across PvDBP II . Omega parameter estimates (ω) calculated using omegaMap. ω > 1 is evidence for diversifying natural selection, whereas ω < 1 is evidence for purifying natural selection. The sitewise mean (solid line) and 95% HPD intervals (dotted lines) are shown (A). The short lines above the solid line represent previously identified epitopes: 5 [299-VNNTDTNFH(R/S)DITFR-313], 16 [321-LIYDAAVEGDLL(L/F)KL-335], 18 [325-AAVEGDLL(L/F)KLNNYR-339], 20 [329-GDLL(L/F)KLNNYRYNKD-343], 45 [379-SIFGT(D/G)(E/K)(K/N)AQQ(R/H)RKQ-393], 48 [385-(E/K)(K/N)AQQ(R/H)RKQWWNESK-399] and 66 [421-ICK(L/I)NV(A/P)VNIEPQIY-435] [30, 49] and in silico predicted promiscuous epitopes: Ia [416-G(N/K)F(I/M)WICK(L/I)-424], Ib [482-KSYD(Q/E)WITR-490], Ic [497-VLSNKF(I/K)SVKNAEK-510], IIa [408-YSVKKRLKG(N/K)-417] and IIb [418-F(I/M)WICK(L/I)NV-426]. (B) Nucleotide diversity (π) and standard deviation (SD) were calculated for each epitope sequence. Epitope nucleotide diversity values significantly higher than the global nucleotide diversity of PvDBPII (π = 0.0081) are shown in bold.