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Figure 1 | Malaria Journal

Figure 1

From: Dynamic subcellular localization of isoforms of the folate pathway enzyme serine hydroxymethyltransferase (SHMT) through the erythrocytic cycle of Plasmodium falciparum

Figure 1

Specificity of the polyclonal anti-PfSHMT preparations. Full-length His-tagged recombinant protein (500 ng) expressed from the genes encoding PfSHMTc (PFL1720w) and PfSHMTm (Pf14_0534) probed on western blots with (A) anti-polyhistidine IgG, (B) the anti-PfSHMTc and (C) the anti-PfSHMTm preparations used for subsequent immunofluorescence studies. Panels D and E are western blots of total parasite extracts from K1 and 3D7 probed with anti-PfSHMTc (D) and anti-PfSHMTm (E). Rc, recombinant PfSHMTc; Rm, recombinant PfSHMTm; M, prestained molecular weight markers.

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Malaria Journal

ISSN: 1475-2875

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